Alanine-scanning mutagenesis reveals residues involved in binding of pap-3-encoded pili.
In order to identify functionally important residues in the pap-3-encoded adhesin, oligonucleotide-directed mutagenesis was used to substitute alanine(s) at sixteen positions in the adhesion. These alanine substitutions span nearly every domain and hydrophilic peak of the protein. The effects of the...
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| 發表在: | J Bacteriol |
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| Principais autores: | , , , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
American Society for Microbiology (ASM)
1994
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC205354/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7908904/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.176.8.2312-2317.1994 |
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