Alanine-scanning mutagenesis reveals residues involved in binding of pap-3-encoded pili.
In order to identify functionally important residues in the pap-3-encoded adhesin, oligonucleotide-directed mutagenesis was used to substitute alanine(s) at sixteen positions in the adhesion. These alanine substitutions span nearly every domain and hydrophilic peak of the protein. The effects of the...
Saved in:
| Published in: | J Bacteriol |
|---|---|
| Main Authors: | , , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
American Society for Microbiology (ASM)
1994
|
| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC205354/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7908904/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.176.8.2312-2317.1994 |
| Tags: |
No Tags, Be the first to tag this record!
|