Alanine-scanning mutagenesis reveals residues involved in binding of pap-3-encoded pili.

In order to identify functionally important residues in the pap-3-encoded adhesin, oligonucleotide-directed mutagenesis was used to substitute alanine(s) at sixteen positions in the adhesion. These alanine substitutions span nearly every domain and hydrophilic peak of the protein. The effects of the...

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Detalles Bibliográficos

Publicado en:	J Bacteriol
Principais autores:	Klann, A G, Hull, R A, Palzkill, T, Hull, S I
Formato:	Artigo
Idioma:	Inglês
Publicado:	American Society for Microbiology (ASM) 1994
Assuntos:	Research Article
Acceso en liña:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC205354/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7908904/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.176.8.2312-2317.1994
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