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Creation of libraries with long ORFs by polymerization of a microgene

We describe a novel method for constructing pools of DNA sequences that encode large proteins with molecular diversity. Sets of primer pairs that form 8 to 10 complementary base pairs in the 3′ region and have double mismatch pairs at their 3′-OH ends were designed so that primer dimers recreated sh...

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Bibliografiske detaljer
Main Authors: Shiba, Kiyotaka, Takahashi, Yuki, Noda, Tetsuo
Format: Artigo
Sprog:Inglês
Udgivet: The National Academy of Sciences of the USA 1997
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC20522/
https://ncbi.nlm.nih.gov/pubmed/9108059
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