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Creation of libraries with long ORFs by polymerization of a microgene
We describe a novel method for constructing pools of DNA sequences that encode large proteins with molecular diversity. Sets of primer pairs that form 8 to 10 complementary base pairs in the 3′ region and have double mismatch pairs at their 3′-OH ends were designed so that primer dimers recreated sh...
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| Auteurs principaux: | , , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
The National Academy of Sciences of the USA
1997
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC20522/ https://ncbi.nlm.nih.gov/pubmed/9108059 |
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