High-efficiency gene inactivation and replacement system for gram-positive bacteria.

A system for high-efficiency single- and double-crossover homologous integration in gram-positive bacteria has been developed, with Lactococcus lactis as a model system. The system is based on a thermosensitive broad-host-range rolling-circle plasmid, pG+host5, which contains a pBR322 replicon for p...

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Detalhes bibliográficos

Publicado no:	J Bacteriol
Principais autores:	Biswas, I, Gruss, A, Ehrlich, S D, Maguin, E
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1993
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC204764/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8501066/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.175.11.3628-3635.1993
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