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Ethanol Extraction Requirement for Purification of Protein Labeled with [(3)H]Leucine in Aquatic Bacterial Production Studies

The trichloroacetic acid (TCA)-insoluble fraction of water column bacteria labeled with [(3)H]leucine contained an ethanol-soluble fraction accounting for up to 44% of the label. A component of the ethanol-soluble fraction is [(3)H]leucine. Labeled-protein purification requires an ethanol wash step....

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Detalhes bibliográficos
Main Authors: Wicks, Richard J., Robarts, Richard D.
Formato: Artigo
Idioma:Inglês
Publicado em: 1988
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC204452/
https://ncbi.nlm.nih.gov/pubmed/16347811
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