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Transfection of Lactobacillus bulgaricus protoplasts by bacteriophage DNA.

A protoplast transfection system has been developed for Lactobacillus bulgaricus. The procedure involves a polyethylene glycol-mediated fusion of bacteriophage DNA encapsulated in liposomes into mutanolysin-treated cells. With L. bulgaricus B004 and DNA isolated from the phage phi c5004, transfectio...

Ausführliche Beschreibung

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Bibliografische Detailangaben
Veröffentlicht in:Appl Environ Microbiol
Hauptverfasser: Boizet, B, Flickinger, J L, Chassy, B M
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology (ASM) 1988
Schlagworte:
Online-Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC204420/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3066292/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.54.12.3014-3018.1988
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