Transfection of Lactobacillus bulgaricus protoplasts by bacteriophage DNA.

A protoplast transfection system has been developed for Lactobacillus bulgaricus. The procedure involves a polyethylene glycol-mediated fusion of bacteriophage DNA encapsulated in liposomes into mutanolysin-treated cells. With L. bulgaricus B004 and DNA isolated from the phage phi c5004, transfectio...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Boizet, B, Flickinger, J L, Chassy, B M
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1988
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC204420/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3066292/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.54.12.3014-3018.1988
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