Rapid and simple method for double staining of bacteria with 4',6-diamidino-2-phenylindole and fluorescein isothiocyanate-labeled antibodies.
Bacteria were either heat fixed on microscope slides or filtered with 0.2 micron-pore-size Nuclepore filters. The samples were stained with 4',6-diamidino-2-phenylindole (DAPI) for total staining and with polyvalent rabbit antibodies and fluorescein isothiocyanate-conjugated swine anti-rabbit antibo...
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| Published in: | Appl Environ Microbiol |
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| Main Author: | |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
American Society for Microbiology (ASM)
1988
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC204409/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2464967/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.54.12.2949-2952.1988 |
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