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Rapid and simple method for double staining of bacteria with 4',6-diamidino-2-phenylindole and fluorescein isothiocyanate-labeled antibodies.

Bacteria were either heat fixed on microscope slides or filtered with 0.2 micron-pore-size Nuclepore filters. The samples were stained with 4',6-diamidino-2-phenylindole (DAPI) for total staining and with polyvalent rabbit antibodies and fluorescein isothiocyanate-conjugated swine anti-rabbit antibo...

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Vydáno v:Appl Environ Microbiol
Hlavní autor: Hoff, K A
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Microbiology (ASM) 1988
Témata:
On-line přístup:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC204409/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2464967/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.54.12.2949-2952.1988
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