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Increased production of aspartase in Escherichia coli K-12 by use of stabilized aspA recombinant plasmid.

Recombinant plasmid pYT471, which consists of the aspartase gene (aspA) and the multicopy vector pBR322, was lost from cells of Escherichia coli K-12 at high frequencies in medium in which aspartase was abundantly formed due to release from catabolite repression. This plasmid loss was not completely...

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Bibliographic Details
Main Authors: Nishimura, N, Komatsubara, S, Kisumi, M
Format: Artigo
Language:Inglês
Published: 1987
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC204201/
https://ncbi.nlm.nih.gov/pubmed/3324966
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