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An Evaluation of Methods for the Reconstitution of Cytochromes P450 and NADPH P450 Reductase into Lipid Vesicles

Two methods (cholate dialysis and cholate gel filtration) used to incorporate cytochromes P450 and reductase into unilamellar phospholipid vesicles were compared to a standard reconstituted system (SRS) where the proteins were reconstituted with preformed liposomes. Both cholate dialysis and gel fil...

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Autori principali: Reed, James R., Kelley, Rusty W., Backes, Wayne L.
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2006
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2041853/
https://ncbi.nlm.nih.gov/pubmed/16415125
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1124/dmd.105.006825
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