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Effect of Proteolytic Enzymes on Transfection and Transformation of Streptococcus lactis Protoplasts

With both chymotrypsin and mutanolysin used to form protoplasts, consistent transformation frequencies of 10(4) to 10(5) transformants and transfectants per μg of DNA were achieved. The procedure was used to transform protoplasts of Streptococcus cremoris CS224 at low frequency (5 transformants per...

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Hlavní autoři: Woskow, Steven A., Kondo, Jeffery K.
Médium: Artigo
Jazyk:Inglês
Vydáno: 1987
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC204149/
https://ncbi.nlm.nih.gov/pubmed/16347474
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