A Rapid Procedure for Purifying Pectate Lyase from Erwinia carotovora Based on Substrate Affinity
To purify pectate lyase produced by Erwinia carotovora subsp. carotovora, we used the supernatant from 48-h-old cultures grown in broth containing sodium polypectate and yeast extract. The supernatant was combined with the enzyme substrates sodium polypectate and polygalacturonic acid, which were th...
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| 出版年: | Appl Environ Microbiol |
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| 主要な著者: | , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
American Society for Microbiology (ASM)
1987
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC203833/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16347348/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.53.5.1189-1191.1987 |
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