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A Rapid Procedure for Purifying Pectate Lyase from Erwinia carotovora Based on Substrate Affinity

To purify pectate lyase produced by Erwinia carotovora subsp. carotovora, we used the supernatant from 48-h-old cultures grown in broth containing sodium polypectate and yeast extract. The supernatant was combined with the enzyme substrates sodium polypectate and polygalacturonic acid, which were th...

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Библиографические подробности
Главные авторы: Ward, L., de Boer, S. H.
Формат: Artigo
Язык:Inglês
Опубликовано: 1987
Предметы:
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC203833/
https://ncbi.nlm.nih.gov/pubmed/16347348
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