A Rapid Procedure for Purifying Pectate Lyase from Erwinia carotovora Based on Substrate Affinity

To purify pectate lyase produced by Erwinia carotovora subsp. carotovora, we used the supernatant from 48-h-old cultures grown in broth containing sodium polypectate and yeast extract. The supernatant was combined with the enzyme substrates sodium polypectate and polygalacturonic acid, which were th...

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Pubblicato in:	Appl Environ Microbiol
Autori principali:	Ward, L., de Boer, S. H.
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 1987
Soggetti:	Physiology and Biotechnology
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC203833/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16347348/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.53.5.1189-1191.1987
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