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High-efficiency transformation of Streptococcus lactis protoplasts by plasmid DNA.

Streptococcus lactis IL1403 protoplasts were transformed by plasmid pIL204 (5.5 kilobases), which conferred erythromycin resistance with an average efficiency of 5 X 10(6) transformants per microgram of supercoiled DNA. The procedure used and transformation efficiencies obtained were close to those...

Ausführliche Beschreibung

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Bibliografische Detailangaben
Veröffentlicht in:Appl Environ Microbiol
Hauptverfasser: Simon, D, Rouault, A, Chopin, M C
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology (ASM) 1986
Schlagworte:
Online-Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC203539/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3092737/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.52.2.394-395.1986
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