Inflammation and NO(x)-induced nitration: Assay for 3-nitrotyrosine by HPLC with electrochemical detection

The identification of (15)N-labeled 3-nitrotyrosine (NTyr) by gas chromatography/mass spectroscopy in protein hydrolyzates from activated RAW 264.7 macrophages incubated with (15)N-l-arginine confirms that nitric oxide synthase (NOS) is involved in the nitration of protein-bound tyrosine (Tyr). An a...

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Detalhes bibliográficos
Main Authors: Shigenaga, Mark K., Lee, Ho H., Blount, Benjamin C., Christen, Stephan, Shigeno, Eric T., Yip, Helena, Ames, Bruce N.
Formato: Artigo
Idioma:Inglês
Publicado em: The National Academy of Sciences of the USA 1997
Assuntos:
Biological Sciences
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC20348/
https://ncbi.nlm.nih.gov/pubmed/9096372
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