Direct immobilization of DNA oligomers onto the amine-functionalized glass surface for DNA microarray fabrication through the activation-free reaction of oxanine

Oxanine having an O-acylisourea structure was explored to see if its reactivity with amino group is useful in DNA microarray fabrication. By the chemical synthesis, a nucleotide unit of oxanine (Oxa-N) was incorporated into the 5′-end of probe DNA with or without the -(CH(2))(n)- spacers (n = 3 and...

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Autors principals: Pack, Seung Pil, Kamisetty, Nagendra Kumar, Nonogawa, Mitsuru, Devarayapalli, Kamakshaiah Charyulu, Ohtani, Kairi, Yamada, Kazunari, Yoshida, Yasuko, Kodaki, Tsutomu, Makino, Keisuke
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 2007
Matèries:
Methods Online
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2034461/
https://ncbi.nlm.nih.gov/pubmed/17715142
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkm619
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