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Hybridization of polyuridylic acid to human DNA immobilized onto nitrocellulose filters.

The level of deoxyadenylate (da) regions in human DNA was estimated from formation of poly(U)-poly(da) triplexes on nitrocellulose filters that were RNAase resistant. The (dA) rich sequences were determined following mild ribonuclease treatment of the poly(U)-DNA hybrids (5 mug/ml at 25 degreesC for...

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Bibliographic Details
Main Authors: Pero, R W, Bryngelsson, T, Bryngelsson, C, Deutsch, A, Nordén, A, Norgren, A
Format: Artigo
Language:Inglês
Published: 1975
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC343503/
https://ncbi.nlm.nih.gov/pubmed/1153334
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