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Hybridization of polyuridylic acid to human DNA immobilized onto nitrocellulose filters.
The level of deoxyadenylate (da) regions in human DNA was estimated from formation of poly(U)-poly(da) triplexes on nitrocellulose filters that were RNAase resistant. The (dA) rich sequences were determined following mild ribonuclease treatment of the poly(U)-DNA hybrids (5 mug/ml at 25 degreesC for...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1975
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC343503/ https://ncbi.nlm.nih.gov/pubmed/1153334 |
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