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Cloning and characterization of two genes from Bacillus polymyxa expressing beta-glucosidase activity in Escherichia coli.

DNA fragments from Bacillus polymyxa which encode beta-glucosidase activity were cloned in Escherichia coli by selection of yellow transformants able to hydrolyze the artificial chromogenic substrate p-nitrophenyl-beta-D-glucopyranoside. Restriction endonuclease maps and Southern analysis of the clo...

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Bibliographic Details
Published in:Appl Environ Microbiol
Main Authors: González-Candelas, L, Aristoy, M C, Polaina, J, Flors, A
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 1989
Subjects:
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC203242/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2515802/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.55.12.3173-3177.1989
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