Effects of Ligands on the Mobility of an Active-Site Loop in Tyrosine Hydroxylase as Monitored by Fluorescence Anisotropy

Fluorescence anisotropy has been used to monitor the effect of ligands on a mobile loop over the active site of tyrosine hydroxylase. Phe184 in the center of the loop was mutated to tryptophan, and the three native tryptophan residues were mutated to phenylalanine to form an enzyme with a single try...

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Bibliographic Details
Main Authors: Sura, Giri R., Lasagna, Mauricio, Gawandi, Vijay, Reinhart, Gregory D., Fitzpatrick, Paul F.
Format: Artigo
Language:Inglês
Published: 2006
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2031214/
https://ncbi.nlm.nih.gov/pubmed/16878998
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi060754b
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