Cloning of an endo-1,4-beta-D-glucanase gene from Clostridium josui and its expression in Escherichia coli.

The gene for carboxymethyl cellulose-degrading enzyme (endoglucanase) from Clostridium josui (FERM P-9684) was cloned in Escherichia coli HB101 with pBR322. A 5.6-kilobase-pair HindIII fragment encoding an endoglucanase was hybridized with C. josui chromosomal DNA. The size of the cloned DNA fragmen...

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Veröffentlicht in:	Appl Environ Microbiol
Hauptverfasser:	Ohmiya, K, Fujino, T, Sukhumavasi, J, Shimizu, S
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1989
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC203087/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2679386/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.55.9.2399-2402.1989
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