Mössbauer studies of alkane ω-hydroxylase: Evidence for a diiron cluster in an integral-membrane enzyme

The gene encoding the alkane ω-hydroxylase (AlkB; EC 1.14.15.3) from Pseudomonas oleovorans was expressed in Escherichia coli. The integral-membrane protein was purified as nearly homogeneous protein vesicles by differential ultracentrifugation and HPLC cation exchange chromatography without the det...

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Autores principales: Shanklin, John, Achim, Catalina, Schmidt, Hermann, Fox, Brian G., Münck, Eckard
Formato: Artigo
Lenguaje:Inglês
Publicado: The National Academy of Sciences of the USA 1997
Materias:
Biological Sciences
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC20308/
https://ncbi.nlm.nih.gov/pubmed/9096332
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