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Single-strand DNA intermediates in phage λ’s Red recombination pathway

An assay was developed to assess early intermediates arising in λ’s Red recombination pathway. Double-strand breaks were delivered in vivo to nonreplicating λ chromosomes. Analysis by blot hybridization of total DNA extracts revealed the following: (i) long (>1.4 kilobases) single-strand DNA (ssDNA)...

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Veröffentlicht in:Proc Natl Acad Sci U S A
Hauptverfasser: Hill, Stuart A., Stahl, Mary M., Stahl, Franklin W.
Format: Artigo
Sprache:Inglês
Veröffentlicht: National Academy of Sciences 1997
Schlagworte:
Online-Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC20303/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9096327/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.94.7.2951
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