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Single-strand DNA intermediates in phage λ’s Red recombination pathway

An assay was developed to assess early intermediates arising in λ’s Red recombination pathway. Double-strand breaks were delivered in vivo to nonreplicating λ chromosomes. Analysis by blot hybridization of total DNA extracts revealed the following: (i) long (>1.4 kilobases) single-strand DNA (ssD...

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Detalles Bibliográficos
Main Authors: Hill, Stuart A., Stahl, Mary M., Stahl, Franklin W.
Formato: Artigo
Idioma:Inglês
Publicado: The National Academy of Sciences of the USA 1997
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC20303/
https://ncbi.nlm.nih.gov/pubmed/9096327
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