Cloning and expression in Escherichia coli of the Azospirillum brasilense Sp7 gene encoding ampicillin resistance.

The Azospirillum brasilense ATCC 29145 gene coding for beta-lactamase was cloned in Escherichia coli. The gene was expressed in E. coli from its own promoter as a 30-kilodalton protein, conferring resistance to high levels of beta-lactam antibiotics. The DNA sequence containing the beta-lactamase ge...

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Détails bibliographiques
Auteurs principaux: Verreth, C, Cammue, B, Swinnen, P, Crombez, D, Michielsen, A, Michiels, K, Van Gool, A, Vanderleyden, J
Format: Artigo
Langue:Inglês
Publié: 1989
Sujets:
Research Article
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC203002/
https://ncbi.nlm.nih.gov/pubmed/2675764
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