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Cloning and expression in Escherichia coli of the Azospirillum brasilense Sp7 gene encoding ampicillin resistance.

The Azospirillum brasilense ATCC 29145 gene coding for beta-lactamase was cloned in Escherichia coli. The gene was expressed in E. coli from its own promoter as a 30-kilodalton protein, conferring resistance to high levels of beta-lactam antibiotics. The DNA sequence containing the beta-lactamase ge...

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Udgivet i:Appl Environ Microbiol
Principais autores: Verreth, C, Cammue, B, Swinnen, P, Crombez, D, Michielsen, A, Michiels, K, Van Gool, A, Vanderleyden, J
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1989
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC203002/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2675764/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.55.8.2056-2060.1989
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