Cloning and Gene Replacement Mutagenesis of a Pseudomonas atlantica Agarase Gene

An agarase gene (agrA) was isolated by cloning genomic DNA prepared from Pseudomonas atlantica. The agarase activity in recombinant Escherichia coli was found in cell-free culture supernatants and could pass through a 0.45-μm-pore-size membrane separating cells from agar, suggesting that the gene pr...

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Dades bibliogràfiques

Publicat a:	Appl Environ Microbiol
Autors principals:	Belas, Robert, Bartlett, Douglas, Silverman, Michael
Format:	Artigo
Idioma:	Inglês
Publicat:	American Society for Microbiology (ASM) 1988
Matèries:	Genetics and Molecular Biology
Accés en línia:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC202392/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16347536/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.54.1.30-37.1988
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