Cloning and Gene Replacement Mutagenesis of a Pseudomonas atlantica Agarase Gene
An agarase gene (agrA) was isolated by cloning genomic DNA prepared from Pseudomonas atlantica. The agarase activity in recombinant Escherichia coli was found in cell-free culture supernatants and could pass through a 0.45-μm-pore-size membrane separating cells from agar, suggesting that the gene pr...
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| Опубліковано в:: | Appl Environ Microbiol |
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| Автори: | , , |
| Формат: | Artigo |
| Мова: | Inglês |
| Опубліковано: |
American Society for Microbiology (ASM)
1988
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| Предмети: | |
| Онлайн доступ: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC202392/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16347536/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.54.1.30-37.1988 |
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