In vivo labeling of Escherichia coli cell envelope proteins with N-hydroxysuccinimide esters of biotin.

The primary amine coupling reagents succinimidyl-6-biotinamido-hexanoate (NHS-A-biotin) and sulfosuccinimidyl-6-biotinamido-hexanoate (NHS-LC-biotin) were tested for their ability to selectively label Escherichia coli cell envelope proteins in vivo. Probe localization was determined by examining mem...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Bradburne, J A, Godfrey, P, Choi, J H, Mathis, J N
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1993
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC202171/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8480997/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.59.3.663-668.1993
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