In vivo labeling of Escherichia coli cell envelope proteins with N-hydroxysuccinimide esters of biotin.
The primary amine coupling reagents succinimidyl-6-biotinamido-hexanoate (NHS-A-biotin) and sulfosuccinimidyl-6-biotinamido-hexanoate (NHS-LC-biotin) were tested for their ability to selectively label Escherichia coli cell envelope proteins in vivo. Probe localization was determined by examining mem...
محفوظ في:
| الحاوية / القاعدة: | Appl Environ Microbiol |
|---|---|
| المؤلفون الرئيسيون: | , , , |
| التنسيق: | Artigo |
| اللغة: | Inglês |
| منشور في: |
American Society for Microbiology (ASM)
1993
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| الموضوعات: | |
| الوصول للمادة أونلاين: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC202171/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8480997/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.59.3.663-668.1993 |
| الوسوم: |
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