PCR-based preparation of 23S rRNA-targeted group-specific polynucleotide probes.

DNA coding for a variable region within domain III of bacterial 23S rRNA was used as the target for group-specific polynucleotide hybridization probes. The corresponding rDNA was amplified in vitro by the PCR technique in combination with a pair of primers specific for flanking conserved target site...

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Bibliographic Details
Main Authors: Ludwig, W, Dorn, S, Springer, N, Kirchhof, G, Schleifer, K H
Format: Artigo
Language:Inglês
Published: 1994
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Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC201794/
https://ncbi.nlm.nih.gov/pubmed/7524442
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