PCR-based preparation of 23S rRNA-targeted group-specific polynucleotide probes.

DNA coding for a variable region within domain III of bacterial 23S rRNA was used as the target for group-specific polynucleotide hybridization probes. The corresponding rDNA was amplified in vitro by the PCR technique in combination with a pair of primers specific for flanking conserved target site...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Ludwig, W, Dorn, S, Springer, N, Kirchhof, G, Schleifer, K H
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1994
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC201794/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7524442/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.60.9.3236-3244.1994
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