In situ detection of hepatitis A virus in cell cultures and shellfish tissues.

An in situ transcription method was developed to detect hepatitis A virus RNA in both cell cultures and shellfish tissues. Radiolabeled cDNA copies were synthesized in situ by reverse transcriptase-directed transcription after annealing with a specific primer to the viral RNA. Both tritium (3H) and...

Fuld beskrivelse

Bibliografiske detaljer

Udgivet i:	Appl Environ Microbiol
Principais autores:	Romalde, J L, Estes, M K, Szücs, G, Atmar, R L, Woodley, C M, Metcalf, T G
Format:	Artigo
Sprog:	Inglês
Udgivet:	American Society for Microbiology (ASM) 1994
Fag:	Research Article
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC201581/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8031087/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.60.6.1921-1926.1994
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