In situ detection of hepatitis A virus in cell cultures and shellfish tissues.

An in situ transcription method was developed to detect hepatitis A virus RNA in both cell cultures and shellfish tissues. Radiolabeled cDNA copies were synthesized in situ by reverse transcriptase-directed transcription after annealing with a specific primer to the viral RNA. Both tritium (3H) and...

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Bibliografiske detaljer
Main Authors: Romalde, J L, Estes, M K, Szücs, G, Atmar, R L, Woodley, C M, Metcalf, T G
Format: Artigo
Sprog:Inglês
Udgivet: 1994
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC201581/
https://ncbi.nlm.nih.gov/pubmed/8031087
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