Quantitative cell lysis of indigenous microorganisms and rapid extraction of microbial DNA from sediment.

This study reports improvements in two of the key steps, lysis of indigenous cells and DNA purification, required for achieving a rapid nonselective protocol for extracting nucleic acids directly from sodium dodecyl sulfate (SDS)-treated sediment rich in organic matter. Incorporation of bead-mill ho...

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Bibliografiske detaljer
Main Authors: Moré, M I, Herrick, J B, Silva, M C, Ghiorse, W C, Madsen, E L
Format: Artigo
Sprog:Inglês
Udgivet: 1994
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC201519/
https://ncbi.nlm.nih.gov/pubmed/8017936
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