Quantitative cell lysis of indigenous microorganisms and rapid extraction of microbial DNA from sediment.

This study reports improvements in two of the key steps, lysis of indigenous cells and DNA purification, required for achieving a rapid nonselective protocol for extracting nucleic acids directly from sodium dodecyl sulfate (SDS)-treated sediment rich in organic matter. Incorporation of bead-mill ho...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Moré, M I, Herrick, J B, Silva, M C, Ghiorse, W C, Madsen, E L
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1994
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC201519/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8017936/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.60.5.1572-1580.1994
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