Preparation of viable single cell suspensions of tracheal epithelial cells.

This paper reports a procedure used for isolating the entire epithelial lining of the rat trachea. Isolated trachea was initially filled with 0.2% hyaluronidase and incubated at 37 degrees C for 30 min. Tracheas were flushed with medium and then reinflated with 0.5 microgram/ml cytochalasin B and re...

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Main Authors: Johnson, N. F., Margiotta, E. A., Wilson, J. S., Sebring, R. J., Smith, D. M.
Format: Artigo
Jezik:Inglês
Izdano: 1987
Teme:
Research Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2013010/
https://ncbi.nlm.nih.gov/pubmed/3555592
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