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Preparing Highly Viable Single-Cell Suspensions from Mouse Pancreatic Islets for Single-Cell RNA Sequencing

Pancreatic islets consist of several cell types, including alpha, beta, delta, epsilon, and PP cells. Due to cellular heterogeneity, it is challenging to interpret whole-islet transcriptome data. Single-cell transcriptomics offers a powerful method for investigating gene expression at the single-cel...

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Detalhes bibliográficos
Publicado no:STAR Protoc
Main Authors: Lee, Hugo, Engin, Feyza
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7757316/
https://ncbi.nlm.nih.gov/pubmed/33377038
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100144
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