Establishment of an Arbitrary PCR for Rapid Identification of Tn917 Insertion Sites in Staphylococcus epidermidis: Characterization of Biofilm-Negative and Nonmucoid Mutants

Transposon mutagenesis with the Enterococcus faecalis transposon Tn917 is a genetic approach frequently used to identify genes related with specific phenotypes in gram-positive bacteria. We established an arbitrary PCR for the rapid and easy identification of Tn917 insertion sites in Staphylococcus...

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Detalhes bibliográficos
Main Authors: Knobloch, Johannes K.-M., Nedelmann, Max, Kiel, Kathrin, Bartscht, Katrin, Horstkotte, Matthias A., Dobinsky, Sabine, Rohde, Holger, Mack, Dietrich
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2003
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Methods
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC201197/
https://ncbi.nlm.nih.gov/pubmed/14532029
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.69.10.5812-5818.2003
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