Establishment of an Arbitrary PCR for Rapid Identification of Tn917 Insertion Sites in Staphylococcus epidermidis: Characterization of Biofilm-Negative and Nonmucoid Mutants

Transposon mutagenesis with the Enterococcus faecalis transposon Tn917 is a genetic approach frequently used to identify genes related with specific phenotypes in gram-positive bacteria. We established an arbitrary PCR for the rapid and easy identification of Tn917 insertion sites in Staphylococcus...

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Publicat a:	Appl Environ Microbiol
Autors principals:	Knobloch, Johannes K.-M., Nedelmann, Max, Kiel, Kathrin, Bartscht, Katrin, Horstkotte, Matthias A., Dobinsky, Sabine, Rohde, Holger, Mack, Dietrich
Format:	Artigo
Idioma:	Inglês
Publicat:	American Society for Microbiology (ASM) 2003
Matèries:	Methods
Accés en línia:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC201197/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/14532029/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.69.10.5812-5818.2003
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