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Evaluation of five DNA extraction methods for purification of DNA from atherosclerotic tissue and estimation of prevalence of Chlamydia pneumoniae in tissue from a Danish population undergoing vascular repair

BACKGROUND: To date PCR detection of Chlamydia pneumoniae DNA in atherosclerotic lesions from Danish patients has been unsuccessful. To establish whether non-detection was caused by a suboptimal DNA extraction method, we tested five different DNA extraction methods for purification of DNA from ather...

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Udgivet i:BMC Microbiol
Principais autores: Mygind, Tina, Østergaard, Lars, Birkelund, Svend, Lindholt, Jes S, Christiansen, Gunna
Format: Artigo
Sprog:Inglês
Udgivet: BMC 2003
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC201026/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12952556/
https://ncbi.nlm.nih.govhttps://doi.org/10.1186/1471-2180-3-19
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