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Evaluation of five DNA extraction methods for purification of DNA from atherosclerotic tissue and estimation of prevalence of Chlamydia pneumoniae in tissue from a Danish population undergoing vascular repair

BACKGROUND: To date PCR detection of Chlamydia pneumoniae DNA in atherosclerotic lesions from Danish patients has been unsuccessful. To establish whether non-detection was caused by a suboptimal DNA extraction method, we tested five different DNA extraction methods for purification of DNA from ather...

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Main Authors: Mygind, Tina, Østergaard, Lars, Birkelund, Svend, Lindholt, Jes S, Christiansen, Gunna
Format: Artigo
Jezik:Inglês
Izdano: BioMed Central 2003
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC201026/
https://ncbi.nlm.nih.gov/pubmed/12952556
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2180-3-19
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