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Unveiling hidden catalytic contributions of the conserved His/Trp-III in tyrosine recombinases: assembly of a novel active site in Flp recombinase harboring alanine at this position

The catalytic pentad of tyrosine recombinases, that assists the tyrosine nucleophile, includes a conserved histidine/tryptophan (His/Trp-III). Flp and Cre harbor tryptophan at this position; most of their kin recombinases display histidine. Contrary to the conservation rule, Flp(W330F) is a much str...

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Detalhes bibliográficos
Main Authors: Ma, Chien-Hui, Kwiatek, Agnieszka, Bolusani, Swetha, Voziyanov, Yuri, Jayaram, Makkuni
Formato: Artigo
Idioma:Inglês
Publicado em: 2007
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2002523/
https://ncbi.nlm.nih.gov/pubmed/17367810
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2007.02.022
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