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In vivo selection for the directed evolution of L-rhamnulose aldolase from L-rhamnulose-1-phosphate aldolase (RhaD)
Dihydroxyacetone phosphate (DHAP) dependent aldolases have been widely used for organic synthesis. The major drawback of DHAP-dependent aldolases is their strict donor substrate specificity toward DHAP, which is expensive and unstable. Here we report the development of an in vivo selection system fo...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2007
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1992742/ https://ncbi.nlm.nih.gov/pubmed/17572092 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bmc.2007.05.062 |
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