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Generation of Ski-knockdown mice by expressing a long double-strand RNA from an RNA polymerase II promoter

We have developed a new vector, named pDECAP, to express long double-strand RNA (ds-RNA) from an RNA polymerase II (Pol II) promoter. Because the transcripts from pDECAP lack both the 5′-cap structure and the 3′-poly(A) tail that facilitate ds-RNA export to the cytoplasm, long ds-RNA from pDECAP doe...

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Udgivet i:Genes Dev
Principais autores: Shinagawa, Toshie, Ishii, Shunsuke
Format: Artigo
Sprog:Inglês
Udgivet: Cold Spring Harbor Laboratory Press 2003
Fag:
Research Communications
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC196066/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12782652/
https://ncbi.nlm.nih.govhttps://doi.org/10.1101/gad.1073003
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