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Generation of Ski-knockdown mice by expressing a long double-strand RNA from an RNA polymerase II promoter
We have developed a new vector, named pDECAP, to express long double-strand RNA (ds-RNA) from an RNA polymerase II (Pol II) promoter. Because the transcripts from pDECAP lack both the 5′-cap structure and the 3′-poly(A) tail that facilitate ds-RNA export to the cytoplasm, long ds-RNA from pDECAP doe...
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| Autores principales: | , |
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| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
Cold Spring Harbor Laboratory Press
2003
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC196066/ https://ncbi.nlm.nih.gov/pubmed/12782652 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gad.1073003 |
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