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Generation of Ski-knockdown mice by expressing a long double-strand RNA from an RNA polymerase II promoter

We have developed a new vector, named pDECAP, to express long double-strand RNA (ds-RNA) from an RNA polymerase II (Pol II) promoter. Because the transcripts from pDECAP lack both the 5′-cap structure and the 3′-poly(A) tail that facilitate ds-RNA export to the cytoplasm, long ds-RNA from pDECAP doe...

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Autori principali: Shinagawa, Toshie, Ishii, Shunsuke
Natura: Artigo
Lingua:Inglês
Pubblicazione: Cold Spring Harbor Laboratory Press 2003
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC196066/
https://ncbi.nlm.nih.gov/pubmed/12782652
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gad.1073003
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