Use of degenerate primers for polymerase chain reaction cloning and sequencing of the Lactococcus lactis subsp. lactis recA gene.

Two particularly well-conserved stretches in the RecA protein sequences were chosen as templates to synthesize degenerate oligonucleotides, which were used in polymerase chain reaction to amplify an internal recA DNA fragment of Lactococcus lactis subsp. lactis ML3. Using this fragment, we recovered...

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Détails bibliographiques

Publié dans:	Appl Environ Microbiol
Auteurs principaux:	Duwat, P, Ehrlich, S D, Gruss, A
Format:	Artigo
Langue:	Inglês
Publié:	American Society for Microbiology (ASM) 1992
Sujets:	Research Article
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC195839/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1514816/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.58.8.2674-2678.1992
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