Use of degenerate primers for polymerase chain reaction cloning and sequencing of the Lactococcus lactis subsp. lactis recA gene.

Two particularly well-conserved stretches in the RecA protein sequences were chosen as templates to synthesize degenerate oligonucleotides, which were used in polymerase chain reaction to amplify an internal recA DNA fragment of Lactococcus lactis subsp. lactis ML3. Using this fragment, we recovered...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Duwat, P, Ehrlich, S D, Gruss, A
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1992
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC195839/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1514816/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.58.8.2674-2678.1992
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