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Dual staining of natural bacterioplankton with 4',6-diamidino-2-phenylindole and fluorescent oligonucleotide probes targeting kingdom-level 16S rRNA sequences.

A method for quantifying eubacterial cell densities in dilute communities of small bacterioplankton is presented. Cells in water samples were stained with 4',6-diamidino-2-phenylindole (DAPI), transferred to gelatin-coated slides, and hybridized with rhodamine-labeled oligonucleotide probes specific...

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Dettagli Bibliografici
Pubblicato in:Appl Environ Microbiol
Autori principali: Hicks, R E, Amann, R I, Stahl, D A
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology (ASM) 1992
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Accesso online:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC195749/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1379029/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.58.7.2158-2163.1992
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